ISO/TS TECHNICAL SPECIFICATION 20787 First edition 2017-11-23 Nanotechnologies - Aquatic toxicity assessment of manufactured nanomaterials in saltwater lakes using Artemia sp. Nauplii Nanotechnologies-Evaluation de la toxicite des nanomateriaux en milieu aquatique par des Artemia sp Referencenumber IS0/TS 20787:2017(E) so IS02017 IS0/TS20787:2017(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2017,Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior writtenpermission.PermissioncanberequestedfromeitherIsoattheaddressbeloworIso'smemberbodyinthecountryof the requester. IsO copyrightoffice Ch.de Blandonnet 8.CP 401 CH-1214Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +4122749 0947 [email protected] www.iso.org ii IS02017-Allrightsreserved IS0/TS20787:2017(E) Contents Page Foreword .V Introduction. .vi 1 Scope. .1 2 Normative references .1 3 Terms and definitions .2 4 Materials .5 4.1 Test organism 5 4.2 Chemicals. 5 5 Technical equipment 5 5.1 Adequate apparatus for temperature control. 5 5.2 Microscope. 5 5.3 Binocularstereoscope. 5 5.4 Centrifuge. 5 5.5 Air pump. 5 5.6 Singlechannelpipettes 5 5.7 Laboratory balance. 5 5.8 Laboratorywaterpurificationsystem. .6 5.9 Laboratory oven. 6 5.10 Laboratory autoclave. .6 5.11 Centrifuge. 6 5.12 Sonication system. 6 5.13 Hot plate stirrer. 6 5.14 Oxygen meter. 6 5.15 Thermometer... 6 5.16 pHmeter. 6 5.17 Salt meter (salinity meter). .6 5.18 Multiparameterphotometer. .6 5.19 Light source.. 6 5.20 Adequate apparatus for the control of the lighting regime and measurement of light intensity (lux meter). .6 5.21 Equipment for the determination oftotal organic carbon. 6 5.22 Equipment for the determination of chemical oxygen demand (CoD) .6 6 Preparation and characterization of dispersion of nanomaterial .6 6.1 Dispersion preparation. 6 6.2 Dispersioncharacterization 7 6.3 Dispersion stabilityin stocksuspension .7 6.4 Dispersion stabilityin artificial seawater 7 6.5 Preparation of exposure media for toxicity tests 7 Hatching procedure .7 7.1 General. 7 7.2 Dilution water 7 7.3 Storage of cysts .8 7.4 Disinfection of Artemia sp. cysts .8 7.5 Hatching method of Artemia sp. cysts 8 7.6 Harvesting of naupli .8 7.7 Calculation of hatching percentage. .9 8 Effect of nanomaterial on Artemia sp.nauplii .9 8.1 Test groups and controls .9 8.2 Test concentrations. .9 8.3 Exposurecondition .10 8.4 Duration. .10 IS02017-Allrightsreserved ii