论文标题
RNMT的综合泛伴奏分析：潜在的预后和免疫生物标志物
Integrative Pan-Cancer Analysis of RNMT: a Potential Prognostic and Immunological Biomarker
论文作者
论文摘要
背景：RNA鸟嘌呤-7甲基转移酶（RNMT）是N7-甲基鸟苷的主要调节剂之一，RNMT的放松管制与肿瘤发育和免疫代谢相关。但是，RNMT在Pan-Canter中的特定功能尚不清楚。 方法：使用多个数据库分析了不同癌症中的RNMT表达，包括癌细胞系百科全书（CCLE），基因型 - 组织表达项目（GTEX）和癌症基因组图集（TCGA）。 Cox回归分析和Kaplan-Meier分析用于估计RNMT表达与预后的相关性。该数据还用于研究RNMT表达与常见免疫调节剂，肿瘤突变负担（TMB），微卫星不稳定性（MSI），不匹配修复（MMR）和DNA甲基转移酶（DNMT）。此外，使用CBIOPORTAL网站评估RNMT更改的特征。 TISDB数据库用于获得不同亚型的表达。肿瘤免疫估计资源（计时器）数据库用于分析RNMT与肿瘤免疫浸润之间的关联。基因集富集分析（GSEA）用于识别相关途径。 结果：RNMT在癌症中普遍存在高度表达，生存分析表明，其表达与各种癌症类型的临床预后高度相关。值得注意的是，RNMT参与免疫调节，并在肿瘤微环境中起着至关重要的作用。在结肠腺癌，肾脏肾透明细胞癌和肝肝细胞癌中，RNMT表达与六种免疫细胞类型表达之间存在正相关。此外，RNMT表达与大多数癌症类型的免疫调节剂高度相关，并且与TMB，MSI，MMR和DNMT相关。最后，GSEA表明RNMT可能与肿瘤免疫相关。
Background: RNA guanine-7 methyltransferase (RNMT) is one of the main regulators of N7-methylguanosine, and the deregulation of RNMT correlated with tumor development and immune metabolism. However, the specific function of RNMT in pan-cancer remains unclear. Methods: RNMT expression in different cancers was analyzed using multiple databases, including Cancer Cell Line Encyclopedia (CCLE), Genotype-Tissue Expression Project (GTEx), and The Cancer Genome Atlas (TCGA). Cox regression analysis and Kaplan-Meier analysis were used to estimate the correlation of RNMT expression to prognosis. The data was also used to research the relationship between RNMT expression and common immunoregulators, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), and DNA methyltransferase (DNMT). Additionally, the cBioPortal website was used to evaluate the characteristics of RNMT alteration. The TISDB database was used to obtain the expression of different subtypes. The Tumor Immune Estimation Resource (TIMER) database was used to analyze the association between RNMT and tumor immune infiltration. Gene set enrichment analysis (GSEA) was used to identify the relevant pathways. Results: RNMT was ubiquitously highly expressed across cancers and survival analysis revealed that its expression was highly associated with the clinical prognosis of various cancer types. Remarkably, RNMT participates in immune regulation and plays a crucial part in the tumor microenvironment. A positive association was found between RNMT expression and six immune cell types expression in colon adenocarcinoma, kidney renal clear cell carcinoma, and liver hepatocellular carcinoma. Moreover, RNMT expression was highly associated with immunoregulators in most cancer types, and correlated to TMB, MSI, MMR, and DNMT. Finally, GSEA indicated that RNMT may correlate with tumor immunity.
