论文标题
VACHT和VGLUT3在纹状体胆碱能静脉曲张中的纳米镜分布表明两个转运蛋白在突触囊泡中的共定位和分离
Nanoscopic distribution of VAChT and VGLUT3 in striatal cholinergic varicosities suggests colocalization and segregation of the two transporters in synaptic vesicles
论文作者
论文摘要
纹状体胆碱能中间神经元（CINS）使用乙酰胆碱（ACH）和谷氨酸（GLUT）调节纹状体网络，因为它们表达用于ACH（VACHT）和GLUT（VGLUT3）的囊泡转运蛋白。但是，ACH和GLUT是否同时释放和/或独立于胆碱能静脉曲张是一个悬而未决的问题。该问题的答案需要在单个突触囊泡（SV）水平上对囊泡转运蛋白的多通道检测。在这里，我们使用超分辨率刺激的发射耗尽显微镜（STED）来表征和量化CINS SVS中VACHT和VGLUT3的分布。 VACHT和VGLUT3-免疫荧光斑点之间最近的邻居距离分析表明，34％的CINS SV均包含VACHT和VGLUT3。此外，40％的SV仅表示VACHT，而26％的SV仅包含VGLUT3。这些结果表明，来自CINS的SV具有同时或独立ACH和/或Glut的同时存储。总体而言，这些形态学的发现支持CINS静脉曲张可以用ACH或GLUT发出信号或两者兼而有之的复杂性水平。
Striatal cholinergic interneurons (CINs) use acetylcholine (ACh) and glutamate (Glut) to regulate the striatal network since they express vesicular transporters for ACh (VAChT) and Glut (VGLUT3). However, whether ACh and Glut are released simultaneously and/or independently from cholinergic varicosities is an open question. The answer to that question requires the multichannel detection of vesicular transporters at the level of single synaptic vesicle (SV). Here, we used super-resolution STimulated Emission Depletion microscopy (STED) to characterize and quantify the distribution of VAChT and VGLUT3 in CINs SVs. Nearest-neighbor distances analysis between VAChT and VGLUT3-immunofluorescent spots revealed that 34% of CINs SVs contain both VAChT and VGLUT3. In addition, 40% of SVs expressed only VAChT while 26% of SVs contain only VGLUT3. These results suggest that SVs from CINs have the potential to store simultaneously or independently ACh and/or Glut. Overall, these morphological findings support the notion that CINs varicosities can signal with either ACh or Glut or both with an unexpected level of complexity.
