论文标题
一种微流体方法，用于被动捕获微结构中的精子
A microfluidic method for passive trapping of sperms in microstructures
论文作者
论文摘要
精子运动是男性生育能力的先决条件。通常，通过诸如离心型的侵略性方法来实现辅助生殖技术中的运动精子的浓度 - 用于人类和动物的繁殖。在这里，我们提出了一种被动技术，用于放大运动精子浓度，而没有外部强加的力或流动。该技术是基于概率率，运动单元，进入复杂结构的概率率之间的差异。在微结构设备的微流体实验中证明了该技术的有效性，并比较了不同几何形状中的捕获功率。在这些微陷阱中，我们观察到了接近10的细胞浓度的增强，而在运动和非运动型之间的对比增加了相似的因素。现实几何形状中合适的相互作用模型精子的模拟对实验结果进行了重现，扩展了观测范围并突出显示最佳陷阱设计关键的成分。
Sperm motility is a prerequisite for male fertility. Enhancing the concentration of motile sperms in assisted reproductive technologies - for human and animal reproduction - is typically achieved through aggressive methods such as centrifugation. Here we propose a passive technique for the amplification of motile sperm concentration, with no externally imposed forces or flows. The technique is based upon the disparity between probability rates, for motile cells, of entering in and escaping from complex structures. The effectiveness of the technique is demonstrated in microfluidic experiments with microstructured devices, comparing the trapping power in different geometries. In these micro-traps we observe an enhancement of cells concentration close to 10, with a contrast between motile and non-motile increased by a similar factor. Simulations of suitable interacting model sperms in realistic geometries reproduce quantitatively the experimental results, extend the range of observations and highlight the ingredients that are key to optimal trap design.
