论文标题
血浆蛋白吸附到可生物降解的镁合金的MD模拟和实验，以促进细胞反应
MD simulations and experiments of plasma proteins adsorption to the biodegradable magnesium alloys to facilitate cell response
论文作者
论文摘要
一旦将镁合金植入体内，立即被血浆蛋白覆盖。涂层合金表面促进了成骨细胞的吸附和生长。本文中，对ZK60和AZ31镁合金的体外生物学反应进行了分析，没有血浆蛋白孵育。使用质谱法（MS）对吸附在镁合金上的吸附的血浆蛋白。 MS结果表明，与其他蛋白质相比，与纤维蛋白原，玻璃纤维，纤连蛋白和凝血酶原相关的蛋白质易于吸附在合金表面上的蛋白质。这些蛋白质限制了MG合金的降解并促进骨细胞的吸附和生长，这些蛋白质通过浸入测试和生物相容性测定法证明。此外，还使用分子动力学模拟来分析ZK60和AZ31AT原子水平上纤维蛋白原，纤连蛋白和凝血酶原的吸附的细节。据表明，在材料表面吸附的残基类型对蛋白质的吸附具有重要作用。
Once the magnesium alloy was implanted in the body, it was immediately covered with plasma proteins. The coated alloy surface promoted the adsorption and growth of osteoblasts. Herein, in vitro biological reactions of the ZK60 and AZ31 magnesium alloys were analyzed with and without plasma proteins incubation. The plasma proteins adsorbed on the magnesium alloy were characterized using mass spectrometry (MS). The MS results show that proteins related to bone cells such as fibrinogen, vitronectin, fibronectin, and prothrombin are prone to adsorbed on the surface of the alloys than other proteins. These proteins restrain the degradation of Mg alloys and promote the adsorption and growth of bone cells, which demonstrated by the immersion tests and biocompatibility assays. Furthermore, molecular dynamics simulations were used to analyze the details of the adsorptions of fibrinogen, fibronectin, and prothrombin on ZK60 and AZ31at atomic level. It is revealed that the type of residues adsorbed on the surface of the material has an important effect on protein adsorption.
