论文标题
散射校正三光子显微镜的波前形状
Scattering correcting wavefront shaping for three-photon microscopy
论文作者
论文摘要
三光子（3p）显微镜由于其在深层组织中的出色表现而受到关注。然而，畸变和光散射仍然构成可达到深度范围的高分辨率成像范围的主要局限性之一。在这里，我们显示了以简单的连续优化算法为导向的散射校正波前形状，并在集成的3P荧光信号的引导下。我们展示了散射层背后的聚焦和成像，并研究了不同样品几何形状和反馈非线性的收敛轨迹。此外，我们通过小鼠头骨显示成像，并展示了一种新型的快速估计方案，该方案大大提高了最佳校正的速度。
Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here, we show scattering correcting wavefront shaping with a simple continuous optimization algorithm, guided by the integrated 3P fluorescence signal. We demonstrate focusing and imaging behind scattering layers and investigate convergence trajectories for different sample geometries and feedback non-linearities. Furthermore, we show imaging through a mouse skull and demonstrate a novel fast phase estimation scheme that substantially increases the speed at which the optimal correction can be found.
