论文标题
由不同能量的质子和氦离子微束诱导的DSB-COCI的修复动力学
Repair kinetics of DSB-foci induced by proton and helium ion microbeams of different energies
论文作者
论文摘要
在这项工作中，研究了辐射诱导的53BP1灶的诱导和修复在人脐静脉内皮细胞中，在PTB微膜上用质子和不同能量的α粒子照射。根据不同离子束诱导的53BP1灶的平均数量分析数据。辐射后不同时间发现的53BP1灶的数量表明，焦点的消失遵循一阶动力学。最初产生的焦点的平均数量显示了LET的预期增加。这项工作的最有趣的发现是，绝对数量的持续焦点随着它们的分数而不是让他们的分数增加。此外，与更高的LET的α粒子相比，质子似乎产生了更持久的焦点。这可以看作是实验证据，表明质子在产生严重的DNA病变方面可能更有效，就像其他工作中所示，并且可能不是表征辐射质量的最佳参数
In this work, the induction and repair of radiation-induced 53BP1 foci were studied in human umbilical vein endothelial cells irradiated at the PTB microbeam with protons and α-particles of different energies. The data were analyzed in terms of the mean number of 53BP1 foci induced by the different ion beams. The number of 53BP1 foci found at different times post-irradiation suggests that the disappearance of foci follows first order kinetics. The mean number of initially produced foci shows the expected increase with LET. The most interesting finding of this work is that the absolute number of persistent foci increases with LET but not their fraction. Furthermore, protons seem to produce more persistent foci as compared to α-particles of even higher LET. This may be seen as experimental evidence that protons may be more effective in producing severe DNA lesions, as was already shown in other work, and that LET may not be the best suited parameter to characterize radiation quality
