论文标题
通过单色电子损失光谱法进行纳米级细胞分析的新途径
A New Path to Nanoscale Cellular Analysis with Monochromated Electron Energy-Loss Spectroscopy
论文作者
论文摘要
高空间分辨率振动光谱是生物材料中纳米级组成分析的主要技术之一。在这里，我们提出了一种通过纳米振动电子损失光谱（EELS）在单色扫描透射电子显微镜中分析全细胞生物标本的新方法。使用该技术的空间和光谱分辨率，我们检查了黄瓜茎的血管系统，并从不同空间分辨率的不同细胞区域中识别出清晰的物理和振动特征。此外，使用构成黄瓜茎的单个组件上的第一原理计算，结合了光学和鳗鱼光谱，我们将振动特征的物理机理拆开，并将组成起源直接分配到不同细胞区域的细胞壁和身体上。这些结果表明，单色电子损坏光谱是生物材料化学组成的纳米级空间映射的一种有前途的技术。
High-spatial-resolution vibrational spectroscopy is one of the principal techniques for nanoscale compositional analysis in biological materials. Here, we present a new method for the analysis of whole-cell biological specimens through nanoscale vibrational electron energy-loss spectroscopy (EELS) in the monochromated scanning transmission electron microscope. Using the combined spatial and spectral resolution of the technique, we examine the vascular system of a cucumber stem and identify clear physical and vibrational signatures from the different cellular regions with high spatial resolution. Furthermore, using first-principles calculations combined with optical and EELS spectroscopy on the individual components that make up the cucumber stem, we unravel the physical mechanisms of the vibrational signatures and directly assign compositional origins to the cell walls and bodies of different cellular regions. These results demonstrate that monochromated electron energy-loss spectroscopy is a promising technique for nanoscale spatial mapping of the chemical composition of biological materials.
