论文标题
Immuno-Histo X射线相对比层造影：新的3D成像技术用于分子断层扫描
Immuno-Histo X-ray Phase Contrast Tomography: New 3D imaging technique for molecular tomography
论文作者
论文摘要
遵循整个身体内特定细胞或分子的命运的尚未达到的能力将在理解疾病机制和监测治疗方法的理解方面具有出色的突破。我们的想法是将生物 - 纳米技术推向一个最先进的3D生物医学成像的水平，以提供从整个器官到细胞水平的多尺度成像，从而在整个疾病促成的生物学的生物学环境中都可以在整个细胞水平上进行高分辨率可视化。我们在这里介绍了新的层析成像程序的第一个概念概念，即免疫X射线对比度扫描（XPCT），它结合了最先进的XPCT，该XPCT提供了整个器官的详细图像，并通过基于免疫组织化学的方法识别相关细胞在细胞水平上的分子成像。我们将靶向相关细胞的金属纳米颗粒和单域抗体结合在一起。我们的结果为新一代的3D生物医学X射线成像奠定了基础。
The still unmet ability of following the fate of specific cells or molecules within the whole body would give an outstanding breakthrough in the comprehension of disease mechanisms and in the monitoring of therapeutic approaches. Our idea is to push forward the bio-nanotechnology to a level where it serves the most advanced 3D bio-medical imaging to provide a multi-scale imaging ranging from the whole organ down to the cellular level, enabling high-resolution visualization of disease-relevant cells within the whole disease-altered biological context. We present here the first proof-of-concept of a novel tomography procedure, Immuno-Histo-X-ray Phase Contrast Tomography (XPCT) that combines cutting-edge XPCT, which provides detailed image of the whole organ, with molecular imaging at the cellular level, identifying the relevant cells via an immunohistochemistry-based approach. We combine metal nanoparticles and a single-domain antibody that target relevant cells. Our results lay the foundation for a new generation of 3D-bio-medical X-ray imaging.
