论文标题
绿色荧光蛋白荧光各向异性的压力和温度依赖性
Pressure and temperature dependence of fluorescence anisotropy of Green Fluorescent Protein
论文作者
论文摘要
我们研究了高静水压力和温度对绿色荧光蛋白（GFP）稳态荧光各向异性的影响。我们发现，在恒温下，GFP的荧光各向异性随着压力的增加而降低。在大气压力下，各向异性随温度的升高而降低，但最大程度的压力大于20 MPa。对应于最大各向异性的温度随压力的增加而增加。通过考虑GFP与溶剂的压力温度依赖性粘度的旋转相关时间的变化，我们认为随着压力的粘度增加并不是导致各向异性随压力减少的主要因素。我们的结果表明，随着发色团周围的H键环环境的变化，荧光各向异性的减少可能导致。
We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure. At atmospheric pressure, anisotropy decreases with increasing temperature but exhibits a maximum with temperature for pressure larger than 20 MPa. The temperature corresponding to the maximum of anisotropy increases with increasing pressure. By taking into account of the rotational correlation time changes of GFP with the pressure-temperature dependent viscosity of the solvent, we argue that viscosity increase with pressure is not a major contributing factor to the decrease in anisotropy with pressure. Our results suggest that the decrease of fluorescence anisotropy with pressure may result from changes in H-bonding environment around the chromophore.
