论文标题
人类血小板之间的破裂力以不同程度的激活
Rupture Forces among Human Blood Platelets at different Degrees of Activation
论文作者
论文摘要
关于在激活和聚集过程中血小板之间相互作用的基础的机械知识知之甚少。尽管血栓的强度可能具有重要的生物学意义，但以前没有研究直接测量了不同激活状态下单个血小板相互作用的粘附力。在这里，我们首先通过最大程度地减少表面介导的血小板激活而填充了这一空隙，其次是通过在单个血小板和AFM悬臂之间产生强粘附力，从而防止早期血小板脱离。我们使用不同的血小板激活状态和血小板受体的阻断来测量两个血小板之间的破裂力。发现破裂力与血小板激活程度成比例增加，但随着特定血小板受体的阻滞而减少。单个血小板相互作用的量化为测试和改善新材料的生物相容性提供了主要观点；量化药物对血小板功能的影响；并评估获得/遗传血小板缺陷的机械特征。
Little is known about mechanics underlying the interaction among platelets during activation and aggregation. Although the strength of a blood thrombus has likely major biological importance, no previous study has measured directly the adhesion forces of single platelet-platelet interaction at different activation states. Here, we filled this void first, by minimizing surface mediated platelet activation and second, by generating a strong adhesion force between a single platelet and an AFM cantilever, preventing early platelet detachment. We applied our setup to measure rupture forces between two platelets using different platelet activation states, and blockade of platelet receptors. The rupture force was found to increase proportionally to the degree of platelet activation, but reduced with blockade of specific platelet receptors. Quantification of single platelet-platelet interaction provides major perspectives for testing and improving biocompatibility of new materials; quantifying the effect of drugs on platelet function; and assessing the mechanical characteristics of acquired/inherited platelet defects.
