论文标题
在外阴，阴茎和头颈的鳞状癌中搜索通常的乳头瘤病毒嫌疑人
Searching beyond the usual papillomavirus suspects in squamous carcinomas of the vulva, penis and head and neck
论文作者
论文摘要
人乳头瘤病毒（HPV）参与了肛门生殖器和头颈癌的病因。 HPV DNA患病率与解剖部位大不相同。确实，与其他解剖部位（主要是外阴，阴茎和头颈部）中可归因于HPV的癌症病例的较低分数相比，肛门和癌性癌中病毒DNA患病率的高率与较低的癌症病例相反。在这里，我们分析了2635张福尔马林固定的石蜡嵌入的手术样品，这些手术样品先前使用SPF10/DEIA程序对HPVS DNA的存在进行了阴性，以确定未通过标准分子流行病学方法明确靶向的其他PVS的存在。使用五个Broad PV PCR引物（CP1/2，FAP6064/FAP64，SKF/SKR，MY9/MY11，MFI/MFII）重新分析所有样品。在头部和颈部癌样品（n = 1141）中，我们从两个BETAHPV（即HPV20和HPV21）中恢复了DNA，并从三个皮肤AlphAPV中恢复了DNA，即HPV2，HPV57和HPV61。在外阴鳞状细胞癌样品（n = 902）中，我们发现了一个含有一种皮肤HPV的DNA的样品之一，即HPV2，29个样品中包含来自粘膜HPV的DNA。在阴茎鳞状细胞癌样品（n = 592）中，我们在16个样品中检索了HPV16的DNA。我们的结果首先表明SPF10/DEIA非常敏感，因为我们仅恢复了2.1％（55/2635）的假阴性结果；其次，尽管可以在癌症样本中检测到皮肤HPV的DNA，但其相对贡献仍然很少（0.23％； 6/2635），并且可以被忽略以进行筛查和疫苗接种的目的；第三，他们对恶性肿瘤的贡献不一定是有必要的，需要阐明。
Human Papillomaviruses (HPVs) are involved in the etiology of anogenital and head and neck cancers. The HPV DNA prevalence greatly differs by anatomical site. Indeed, the high rates of viral DNA prevalence in anal and cer-vical carcinomas contrast with the lower fraction of cancer cases attributable to HPVs in other anatomical sites, chiefly the vulva, the penis and head and neck. Here we analyzed 2635 Formalin Fixed Paraffin Embedded surgical samples that had previously tested negative for the presence of HPVs DNA using the SPF10/DEIA procedure, in order to identify the presence of other PVs not explicitly targeted by standard molecular epidemiologic approaches. All samples were reanalyzed using five broad-PV PCR primer sets (CP1/2, FAP6064/FAP64, SKF/SKR, MY9/MY11, MFI/MFII) targeting the main PV main clades. In head and neck carcinoma samples (n = 1141), we recovered DNA from two BetaHPVs, namely HPV20 and HPV21, and from three cutaneous AlphaPVs, namely HPV2, HPV57 and HPV61. In vulvar squamous cell carcinoma samples (n = 902), we found one of the samples containing DNA of one cutaneous HPV, namely HPV2, and 29 samples contained DNA from essentially mucosal HPVs. In penile squamous cell carcinoma samples (n = 592), we retrieved the DNA of HPV16 in 16 samples. Our results show first that the SPF10/DEIA is very sensitive, as we recovered only 2.1% (55/2635) false negative results; second, that although the DNA of cutaneous HPVs can be detected in cancer samples, their relative contribution remains anyway minor (0.23%; 6/2635) and may be neglected for screening and vaccination purposes; and third, their contribution to malignancy is not necessarily warranted and needs to be elucidated.
