论文标题
单分子取向定位显微镜II：性能比较
Single-molecule orientation localization microscopy II: a performance comparison
论文作者
论文摘要
已经开发了各种技术来测量荧光分子的2D和3D和2D和3D方向，其精度比标准的表现显微镜提高了。由于在典型的单分子实验中具有挑战性的信噪比比率，因此必须选择针对特定目标样品进行优化的成像系统。在这项工作中，我们比较了多种最新和常用的方向定位显微镜方法的性能与测量精度的基本限制。我们的分析揭示了各种实验条件和样品几何形状的最佳成像方法。有趣的是，在许多成像方案中，对标准荧光显微镜的简单修改表现出卓越的性能。
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background ratio in typical single-molecule experiments, it is essential to choose an imaging system optimized for the specific target sample. In this work, we compare the performance of multiple state-of-the-art and commonly used methods for orientation localization microscopy against the fundamental limits of measurement precision. Our analysis reveals optimal imaging methods for various experiment conditions and sample geometries. Interestingly, simple modifications to the standard fluorescence microscope exhibit superior performance in many imaging scenarios.
