论文标题
安培测量和动态模型揭示了锌如何改变神经递质释放的机制
Amperometric Measurements and Dynamic Models Reveal a Mechanism for How Zinc Alters Neurotransmitter Release
论文作者
论文摘要
锌是一种可疑的学习和记忆的增强剂，已显示出影响含神经递质囊泡的胞吐释放和储存。使用透射电子显微镜对囊泡密度核心和光晕进行的结构和大小分析与单细胞安培计量学结合，以研究锌处理后诱导的囊泡尺寸变化，并基于与全量释放相反的部分释放的概念将这些变化与理论预测进行比较。这种强大的组合分析方法确定了囊泡结构和胞外动力学之间存在未悬而未决的牢固联系的存在，这些连接可用于解释通过调节神经递质释放Zn 2+调节突触可塑性的机制。
Zinc, a suspected potentiator of learning and memory, is shown to affect exocytotic release and storage in neurotransmitter-containing vesicles. Structural and size analysis of the vesicular dense core and halo using transmission electron microscopy was combined with single-cell amperometry to study the vesicle size changes induced after zinc treatment and to compare these changes to theoretical predictions based on the concept of partial release as opposed to full quantal release. This powerful combined analytical approach establishes the existence of an unsuspected strong link between vesicle structure and exocytotic dynamics which can be used to explain the mechanism of regulation of synaptic plasticity by Zn 2+ through modulation of neurotransmitter release.
