论文标题
不可逆Michaelis-Menten方程的热力学有效性标准
Thermodynamic validity criterion for the irreversible Michaelis-Menten equation
论文作者
论文摘要
酶动力学通常以不可逆的Michaelis-Menten（MM）方程为特征。但是，在开放的化学反应网络（例如代谢途径）中，这种方法可以导致显着的动力学和热力学不一致。基于非平衡化学热力学的最新发展，我们提出了一个仅根据酶催化反应的平衡常数表示的有效性标准。满足后，它保证了不可逆的MM方程生成动力学和热力学数据的能力，这些数据对于合理的浓度范围是可靠的。因此，我们的有效性标准是确保途径可靠的动力学和热力学建模的宝贵工具。我们还表明，它正确识别了糖酵解和克雷布斯循环中所谓的不可逆酶促反应。
Enzyme kinetics is very often characterised by the irreversible Michaelis-Menten (MM) equation. However, in open chemical reaction networks such as metabolic pathways, this approach can lead to significant kinetic and thermodynamic inconsistencies. Based on recent developments in nonequilibrium chemical thermodynamics, we present a validity criterion solely expressed in terms of the equilibrium constant of the enzyme-catalysed reaction. When satisfied, it guarantees the ability of the irreversible MM equation to generate kinetic and thermodynamic data that are quantitatively reliable for reasonable ranges of concentrations. Our validity criterion is thus a precious tool to ensure reliable kinetic and thermodynamic modelling of pathways. We also show that it correctly identifies the so-called irreversible enzymatic reactions in glycolysis and Krebs cycle.
