论文标题
上转换非线性结构化照明显微镜
Upconversion nonlinear structured illumination microscopy
论文作者
论文摘要
通过生物组织进行视频速率超分辨率成像可以可视化和跟踪细胞生物内生物分子的相互作用和运输。结构化照明显微镜可以对生物样品进行广阔的超级分辨率观察,但受到生物组织对光的强烈吸收和散射的限制,从而降低了其成像分辨率。在这里,我们通过生物透明窗口使用灯笼型纳米颗粒报告了一种光子上转换方案,用于宽场超分辨率成像,以近红外和低辐射性非线性结构化照明为特色。我们证明了976 nm激发和800 nm上转换的发射可以减轻畸变。我们发现,来自单纳米颗粒的上转换排放的非线性响应可以有效地产生傅立叶域中所需的高空间频率分量。这些策略导致显微镜的新模式,分辨率为130 nm,激发波长的1/7和1 fps的帧速率。
Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution observation of biological samples but is limited by the strong absorption and scattering of light by biological tissues, which degrades its imaging resolution. Here we report a photon upconversion scheme using lanthanide-doped nanoparticles for wide-field super-resolution imaging through the biological transparent window, featured by near-infrared and low-irradiance nonlinear structured illumination. We demonstrate that the 976 nm excitation and 800 nm up-converted emission can mitigate the aberration. We found that the nonlinear response of upconversion emissions from single nanoparticles can effectively generate the required high spatial frequency components in Fourier domain. These strategies lead to a new modality in microscopy with a resolution of 130 nm, 1/7th of the excitation wavelength, and a frame rate of 1 fps.